In vitro measurement of percentage cell death - two-way ANOVA design with repeated measures, and randomised blocks.

These are measurements of death of infected host cells (as percentage of total cells) upon infection with two strains of bacteria, measured at two time points, in 6 independent experiments. These data repeated-measures data suitable for two-way linear mixed effects modelling with experiment and subjects as random factors.

data_2w_Tdeath

Format

data.frame: 24 obs. of 6 variables:

Experiment

A random factor with 6 levels "e1", "e2"...

Time

A fixed factor with 2 levels: "t100" & "t300".

Time2

A numeric column that allows plotting data on a quantitative "Time" axis. The "Time" column has "factor" type values that should be used for the ANOVA..

Genotype

A fixed factor with 2 levels that we want to compare "WT" & "KO".

Subject

A random factor with 12 levels: "s1", "s2"... These are cell culture wells that were measured at two time points, and indicate "subjects" that underwent repeated-measures within each of 6 experiments. Subject IDs for WT and KO are unique and clearly indicate different wells.

PI

Numerical dependent variable indicating propidium iodide dye uptake as a measure of cell death. These are percentage of dead cells out of total cells plated.